GCGR/CRE-Luc/HEK293
CBP71345
询 价
索取protocol
产品描述
产品数据库
| I. Background | |
|
胰高血糖素是一种胰腺肽激素，作为胰岛素的反调节激素，刺激葡萄糖顺利获得肝脏释放， 维持葡萄糖稳态。GCGR 是一种 B 类 GPCR，最初被描述为与腺苷酸环化酶功能相连的胰 高血糖素结合体。它在维持葡萄糖稳态中起关键作用，因此被认为是治疗糖尿病的一个有 价值的靶点。 |
|
| II. Description | |
|
GCGR CRE-Luc HEK293 报告基因药靶模型很好的模拟了体内 GCGR 的信号转导过程， 原理见下图所示。
|
|
| III. Introduction | |
| Expressed gene: |
GCGR |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin+200 μg/ml Hygromycin B |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for GCGR |
| Transducer: | Gs |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: |
Embryonic kidney
|
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
V. Representative Data Figure 2. Recombinant GCGR/CRE-Luc/HEK293 stably expressing GCGR.  Figure 3. HTRF cAMP Assay with GCGR CRE-Luc HEK293( C21).  |
|
